genotyping of pasteurella multocida ovine and bovine isolates from iran based on pcr-rflp of omph gene

Genotyping of Pasteurella multocida ovine and bovine isolates from Iran based on PCR-RFLP of ompH gene

Pasteurella multocida (P. multocida), A Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. The outer membrane of Gram-negative bacteria contains of many different protein in very high copy numbers. One of the major outer membrane, the H proteins have functional as high immunogenicity and antigenicity. In this study ...

Molecular typing of avian Pasteurella multocida isolates from Iran by PCR-RFLP of OmpH gene

Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment length polymorphism (RFLP) of a species-specific PCR assay. All of the P.multocida isolates were recovered from fowl cholera outbreaks in Northern provinces of Iran. Amplification was based on the gene ompH, encoding a major outer membrane protein (protein H). PCR with ompH primers amplified...

Diversity of caprine and ovine Pasteurella multocida isolates based on 16S rRNA gene sequencing

In this study, to increase information about the relationship between caprine and ovine isolates of Pasteurella multocida, 16S rRNA gene sequencing of 9 goats (5) and sheep (4) isolates were investigated. Also, capsular type and toxAgene presentation was studied in this paper. All isolates, except one, belong to capsular type A, and toxA+ strain distributed among strains were isolated from both...

Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene.

The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by Dr...

LPS-PCR typing of ovine Pasteurella multocida isolates from Iran based on (L1 to L8) outer core biosynthesis loci

Pasteurella multocida isa gram-negative bacterial pathogen that is causative agent of a wide range of diseases in many animal species and humans. Lipopolysaccharides (LPS) are an important virulence factor, minor changes to structure of which can exert dramatic effects on pathogenicity of P. multocida in its host. LPS can be used for the identification and classification of strains with somatic...

Isolation and typing of Pasteurella multocida poultry isolates from Iran